T cell receptors and B cell receptors of COVID-19 patients
Release date:
2020-11-02
Description:
We collected 23 peripheral blood mononuclear cells (PBMCs) samples from COVID-19 patients. We applied a novel dimer avoidance multiplexed polymerase chain reaction next-generation sequencing assay to analyze T (TCR) and B cell receptor (BCR) repertoires. In this study, iR-RepSeq-plus 7-Chain Cassette was used to generate NGS libraries covering all TCR and BCR chains including TCR-beta, -alpha, -delta, -gamma, and BCR-IgH, -IgK, -IgL. All seven chains were amplified in a single assay.
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