1. Home
  2. Browse
  3. PRJCA003775
Accession PRJCA003775
Title T and B cell receptor repertoire of COVID-19 patients
Relevance Medical
Data types Raw sequence reads
Assembly
Organisms Homo sapiens
Description We collected 23 peripheral blood mononuclear cells (PBMCs) samples from COVID-19 patients. We applied a novel dimer avoidance multiplexed polymerase chain reaction next-generation sequencing assay to analyze T (TCR) and B cell receptor (BCR) repertoires. In this study, iR-RepSeq-plus 7-Chain Cassette was used to generate NGS libraries covering all TCR and BCR chains including TCR-beta, -alpha, -delta, -gamma, and BCR-IgH, -IgK, -IgL. All seven chains were amplified in a single assay.
Sample scope Multiisolate
Release date 2020-10-28
Publication
PubMed ID Article title Journal name DOI Year
Longitudinal Analysis of T and B Cell Receptor Repertoire Transcripts Reveal Dynamic Immune Response in COVID-19 Patients Frontiers in Immunology 10.3389/fimmu.2020.582010 2020
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 81661148056 Analysis of antibody repertoires in patients to elucidate the mechanism of antibody response after 2019-nCoV infection
Submitter Ling    Chen  (chen_ling@gibh.ac.cn)
Organization The First Affiliated Hospital of Guangzhou Medical University
Submission date 2020-10-28

Project Data

Resource name Description
BioSample (23)  show -