NGDC - GSA for Human

HRA000433

HRA000433.xlsx

Title:

Integrated single-cell multiomics analysis reveals novel candidate markers for prognosis in human pancreatic ductal adenocarcinoma

Release date:

2021-12-24

Description:

The epigenomic abnormality of pancreatic ductal adenocarcinoma (PDAC) has rarely been investigated due to its strong heterogeneity. Here, we use single-cell multiomics sequencing to simultaneously analyze the DNA methylome, chromatin accessibility, and transcriptome in each individual tumor cell of PDAC patients. We identified normal epithelial cells in the tumor lesion, which have euploid genomes, normal patterns of DNA methylation and chromatin accessibility comparable to those of normal epithelial cells in adjacent normal tissues. We determined that DNA demethylation in cancer cell genome was strongly enriched in heterochromatin regions but depleted in euchromatin regions and observed stronger negative correlations between gene expression and promoter region DNA methylation in cancer cells than in normal epithelial cells. Through integrated analyses, lower expressions of ZNF667 and ZNF667AS1 were identified to connect to poorer prognosis for PDAC patients.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA003818

Study type:

Disease Study

Disease name:

pancreatic ductal adenocarcinoma

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC000073

DAC name:

BIOPIC_TangLab

Contact person:

Tang Fuchou

Email:

tangfuchou@pku.edu.cn

Description:

Laboratory of Stem Cells and Epigenetics

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