HRA000762
Title:
Single-cell exome sequencing reveals multiple subclones in metastatic colorectal carcinoma
Release date:
2021-05-24
Description:
In this study, we characterized the evolutionary pattern of metastatic CRC (mCRC) by analysing bulk and single-cell exome sequencing data of primary and metastatic tumors from 7 CRC patients with liver metastases. Here, 7 CRC patients had bulk WES, and 4 for whom both bulk and single cell sequencing were pursued. Despite low genomic divergence between paired primary and metastatic cancers in the bulk data, single-cell whole-exome sequencing (scWES) data revealed rare mutations and defined two separate cell populations, indicative of the independent evolutionary trajectory between primary and metastatic tumor cells. We further identified 24 metastatic cell-specific mutated genes and validated their functions in cell migration capacity. In summary, scWES revealed rare mutations that failed to be detected by bulk WES. These rare mutations better define the distinct genomic profiles of primary and metastatic tumor cell clones.
Data Accessibility:   
Controlled access Request Data
BioProject:
Study type:
Disease Study
Disease name:
colorectal cancer
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
DAC name:
mCRC data of DanXie Lab
Contact person:
Xie Dan
Email:
danxie@scu.edu.cn
Lab URL:
https://danxielab.net/people/1/index.html
Description:
In this study, we characterized the evolutionary pattern of metastatic CRC (mCRC) by analysing bulk and single-cell exome sequencing data of primary and metastatic tumors from 7 CRC patients with liver metastases. Here, 7 CRC patients had bulk WES, and 4 for whom both bulk and single cell sequencing were pursued.
Individuals & samples
Files
Submitter:   Xie Dan / danxie@scu.edu.cn
Organization:   West China Hospital Sichuan University
Submission date:   2021-03-25