1. Home
  2. Browse
  3. HRA000908
HRA000908
   HRA000908.xlsx
Title:
single-cell RNA sequencing of colon cancer stem cell (CCSCs) xenograft tumor or low-notch activity tumor cell derived from asymmetric division of stem cells
Release date:
2023-06-01
Description:
For colon cancer stem cell xenograft tumor, single-cell RNA-seq were performed by the 10xGenomics platform.CD133+LGR5+ colon cancer stem cell(CCSCs) were subcutaneously injected into nude mice to form xenografts, which were collected and dissociated into single cells for single-cell RNA sequencing.For low-notch activity tumor cell derived from asymmetric division of stem cells, single-cell RNA-seq were performed by the SMART-seq2 platform.High-Notch-activity(GFP+) colon cancer stem cell(CCSCs) were sorted and seeded for dividing once, while low-Notch-activity daughter cells were collected from the dividing duplets for single-cell RNA-seq.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA005357
Study type:
Disease Study
Disease name:
colorectal cancer
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC000564
DAC name:
Bu Lab
Contact person:
bu pengcheng
Email:
bupc@ibp.ac.cn
Description:
Institute of Biophysics, Chinese Academy of Sciences
Individuals & samples
Files
Request Data
Submitter:   bu pengcheng / bupc@ibp.ac.cn
Organization:   Institute of Biophysics, Chinese Academy of Sciences
Submission date:   2021-06-03
Requests:   1