Stage-specific H3K9me3 allocations ensure retrotransposon silencing in human preimplantation embryos
Release date:
2022-07-28
Description:
We profiled high-resolution genome-wide H3K9me3 in human oocytes and pre-implantation embryos. Conserved in mouse, H3K9me3 is significantly enriched in long terminal repeats (LTRs) and is progressively established along with human embryogenesis. Moreover, after zygotic genome activation (ZGA), human embryos also exhibit epigenetic switch from DNA methylation to H3K9me3 to silence temporarily activated retrotransposons. Notably, we discovered two spatial-temporal waves of H3K9me3 deposition at peri-ZGA and post-ZGA, which mediate the silence of distinct LTR families. DUX is identified as the potential transcription factor regulating human peri-ZGA-specific H3K9me3 allocation, while typical H3K9me3-related co-regulators are responsible for the establishment of post-ZGA-specific H3K9me3. Finally, we found that strong bivalency of H3K4me3/H3K9me3 and H3K4me4/H3K27me3 modifications exist in human blastocysts, as nascent priming for lineage differentiation.
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