High-throughput Saturation Mutagenesis Generates a High-affinity Antibody against SARS-CoV-2 Variants using Protein Surface Display Assay on a Human Cell
Release date:
2022-11-15
Description:
As new mutations continue to emerge, the ability of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus to evade the human immune system and neutralizing antibodies remains a huge challenge for vaccine development and antibody research. The majority of neutralizing antibodies have reduced or lost activity against SARS-CoV-2 variants. In this study, we reported a novel protein surface display system on a mammalian cell for obtaining a higher-affinity antibody in high-throughput manner. Using a saturation mutagenesis strategy through integrating microarray-based oligonucleotide synthesis and single-cell screening assay, we generated a group of new antibodies against diverse prevalent SARS-CoV-2 variants through high-throughput screening the human antibody REGN10987 within 2 weeks.
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Individuals & samples
Files
Submitter: Xi Jianzhong / jzxi@pku.edu.cn
Organization: Peking University
Submission date: 2022-11-14
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HRA003432.xlsx