Single-cell RNA-seq combined spatial transcriptomics analysis decipher cell atlas of human ligament and reveal the process of ligament degeneration
Release date:
2024-08-10
Description:
Anterior cruciate ligament degeneration is an important origin of knee osteoarthritis and a common threat to athletic ability. However, the cellular heterogeneity and underlying mechanisms in the degeneration process remain elusive. To systematically identify and localize cell compartments in normal and degenerated ligaments and investigate how cellular compositions, functions, and interactions participated in the ligamental degeneration, we built a cell atlas of the healthy and degenerated human ligament using scRNA-seq and spRNA-seq. We identified new fibroblast subgroups contributed to the disease and mapped out their spatial distribution in the tissue and revealed two stages of the degenerative process. We compared the cellular interactions between different tissue states and identified important signaling pathways may contribute to the disease.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
Individuals & samples
Files
Submitter: FU WEILI / foxwin2008@163.com
Organization: West China Hospital of Sichuan University
Submission date: 2022-12-30
Aspera Command Line
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
HRA003746.xlsx