NGDC - GSA for Human

HRA004917

HRA004917.xlsx

Title:

Single-cell multi-omics sequencing reveals the epigenomic landscapes in human spermatogenesis [NOA3-NOA8 scRNA-seq]

Release date:

2023-07-09

Description:

Here, by simultaneously investigating the chromatin accessibility, DNA methylome and transcriptome landscapes of human spermatogenesis using the modified single-cell chromatin overall omic-scale landscape sequencing approach (Modified scCOOL-seq), we revealed that the transcriptional changes throughout human spermatogenesis were correlated with the dynamic alternations of chromatin accessibility. Remarkably, a round of DNA demethylation was uncovered upon meiosis initiation in human spermatogenesis, which was associated with male meiotic recombination and conserved in human and mouse. And aberrant DNA hypermethylation at recombination hotspots could be detected in leptotene spermatocytes of certain nonobstructive azoospermia patients. Functionally, the intervention of DNA demethylation affected male meiotic recombination. Collectively, this study provides multi-omics landscapes of human spermatogenesis, and offers insights into the association between DNA demethylation and male meiotic recombination.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA017857

Study type:

Disease Study

Disease name:

azoospermia

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC000218

DAC name:

Southern Medical University

Contact person:

Zhao Xiaoyang

Email:

zhaoxiaoyang@smu.edu.cn

Description:

We will comply with the regulations of the Data Access Committee (DAC) to determine whether to release data

Aspera Command Line