Fostering the Transition to Human Ground-State Pluripotency through Precise NFkB/RELA Circuitry Inhibition
Release date:
2023-11-15
Description:
The transition from human primed pluripotency to a naive state offers insight into embryo implantation. We developed a serum-free system, 6iLV, achieving a 90% conversion rate in 12 days. Multi-omics sequencing revealed chromatin accessibility changes, with increased openness at OCT and KLF binding motifs and decreased accessibility at ZIC, RFX, and NFkB/RELA motifs. MicroRNA profiling showed elevated miR-372/373 and miR-182/183 clusters and decreased miR-302 and miR-363/106 cluster expression. Functional experiments demonstrated that miR-372/373 overexpression promoted the transition, while NFkB/RELA overexpression inhibited it. Mechanistically, miR-372/373 suppressed the expression of NFkB/RELA, facilitating the transition.
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Individuals & samples
Files
Submitter: Liu Jing / liu_jing@gibh.ac.cn
Organization: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Submission date: 2023-11-13
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HRA006013.xlsx