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HRA008135
   HRA008135.xlsx
Title:
tRNA RIP data of METTL1-mediated tRNA m7G modification determines B cell responses in systemic autoimmunity
Release date:
2024-10-25
Description:
293T cells were transfected with either an exogenous METTL1 overexpression plasmid or an empty vector control plasmid. At 48 hours post-transfection, cells were subjected to UV crosslinking at 4000*100 µJ/cm2 energy. Cells were then lysed, and anti-FLAG magnetic beads were used for immunoprecipitation of crosslinked RNA-protein complexes for 4 hours at 4°C with rotation. RNA was subsequently extracted from the immunoprecipitated material using TRIzol reagent following the manufacturer's instructions. Recombinant wild-type AlkB and the D135S AlkB mutant proteins were employed to remove dominant methylations from the isolated RNA samples, facilitating efficient reverse transcription of tRNAs.
Data Accessibility:   
Controlled access Request Data
BioProject:
PRJCA022405
Study type:
Cell line related study
Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.


DAC NO.:
HDAC003533
DAC name:
Zhang lab data management
Contact person:
Zhang Hui
Email:
zhangh656@mail.sysu.edu.cn
Description:
management and authorization of sequencing or protein data produced by Zhang lab.
Individuals & samples
Files
Request Data
Submitter:   Zhang Hui / zhangh656@mail.sysu.edu.cn
Organization:   The First Affiliated Hospital, Sun Yat-sen University
Submission date:   2024-07-26
Requests:   -