The analysis of transcriptional changes due to lentiviral vector transfection and overexpression of external gene uncovers disparities in gene expression amounts and alternative splicing in HEK293T
Release date:
2025-02-23
Description:
In this study, we constructed two transfected cell lines from human embryonic kidney 293T cells through lentiviral vector transfection. RNA sequencing was performed, followed by the identification of differentially expressed genes (DEGs) and genes with differential transcript usage (gDTUs) across cell lines.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
Individuals & samples
Files
Submitter: Leng liang / lling@cdutcm.edu.cn
Organization: Chengdu University of Chinese Medicine
Submission date: 2025-02-21
Aspera Command Line
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
HRA010505.xlsx