NGDC - GSA for Human

HRA011960

HRA011960.xlsx

Title:

Transcriptome Analysis Reveals Mechanical Properties of Leukemic Stem Cells in AML.

Release date:

2025-09-30

Description:

To investigate the molecular heterogeneity and dynamic responses of acute myeloid leukemia (AML) subpopulations, we performed a controlled ex vivo perturbation study followed by transcriptional profiling. Peripheral blood or bone marrow samples were collected from de novo AML patients (n=20, diagnosed per WHO criteria). Mononuclear cells were isolated by density gradient centrifugation and subjected to fluorescence-activated cell sorting (FACS) to separate two phenotypically distinct subsets: SMALL cells and LARGE cells. Each purified subset was allocated to experimental arms with specific pharmacological interventions:SMALL cell cohort: Untreated control (n=5) vs. Jasplakinolide (Jas, 100 nM, 6h) treatment (n=5); LARGE cell cohort: Untreated control (n=5) vs. Latrunculin A (Lat A, 5 uM 6h) treatment (n=5); Following 6-hour incubation under standardized culture conditions (37°C, 5% CO2), all four groups (SMALL, SMALL+Jas, LARGE, LARGE+Lat A) were processed for bulk RNA sequencing.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA035387

Study type:

Disease Study

Disease name:

acute myeloid leukemia

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC005369

DAC name:

Hematology immunology

Contact person:

Ni fang

Email:

fangni@ustc.edu.cn

Description:

Blood and Immunology

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