| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection
| Layout |
|
A 200ul aliquot of each SARS2 infected whole-BALF sample was used to extract RNA using Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) and Trizol LS (Thermo Fisher Scientific, Carlsbad, CA, USA) in biosafety III laboratory, the rest samples were operated following the same protocol in biosafety II laboratory. The RNA was then reverse transcribed, and amplified using an Ovation Trio RNA-Seq library preparation kit (NuGEN, CA, USA) |
RNA-Seq |
METATRANSCRIPTOMIC |
unspecified |
FRAGMENT
|
|
