| 实验编号 | CRX017987 |
| 物种名称 | Mus musculus |
| 标题 | 4-cell R2 |
| 项目编号 | PRJCA000241 |
| 样本编号 | SAMC013116 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
In Situ Hi-C library generation was performed with some modification accordingto the procedures described in detail elsewhere (Rao et al., 2014). All samples were crosslinked with 1%formaldehyde at room temperature for 10 minutes. Nuclei were permeabilized. DNAwas digested with 10 units of MboI, and the ends of restriction fragments werelabeled using biotinylated nucleotides and ligated in a very small volume. Thecrosslinks, ligated DNA was pelleted, washed with 10mM Tris buffer once, thenreversed in 10mM Tris buffer and 1.25mg/ml proteinase K (Qiagen, 19133),incubated at 65°C for 5h and 75°C for 30min to inactivate the protease. The reversedDNA was sheared to a length of ~400bp, and directly treated with EndRepair/dA-Tailing Module (NEB, E7442L) and Ligation Module (NEB, E7445L).The biotin-labelled ligation junction DNA was pulled down with streptavidin beadsand amplified for 12-14 cycles. The amplification mixture was purified usingAMPURE XP beads to select size 400-600bp. The result |
OTHER |
GENOMIC |
unspecified |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2017-07-13 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR008688 |
CRD018310
CRD018311
CRD018312
CRD018313
|
40,619.44
45,630.8
40,487.94
46,048.71
|
|
| 提交者 | Xuepeng Chen (chenxp@big.ac.cn) |
|---|
| 所属单位 | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| 提交日期 | 2017-05-31 |
|---|
