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Total RNA was isolated using TRIZOL (Invitrogen, Carlsbad, CA). The RNA concentration and quality were determined with NanoDrop, Qubit and Agilent 2100 instruments. A RiboMinus kit (KAPA, USA) was used to deplete ribosomal RNA and then was incubated at 37 °C with 10 U ?g-1 RNase R (Epicentre, Madison, WI). RNA-/RNAase R-treated samples were used as templates for separate cDNA libraries following the TruSeq protocol (Illumina, San Diego, CA).Finally, the library was sequenced on the Illumina HiSeq 2500 platform at the Research Facility Center at Beijing Institutes of Life Science, CAS, with paired-end 250 bp (PE250) kits. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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