Accession | CRX030768 |
Organism | Homo sapiens |
Title | T7_single cell RNAseq |
BioProject | PRJCA001063 |
BioSample | SAMC047078 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The concentration of single cell suspension was counted using Countess (Thermo) and adjusted to 1000 cells/ul. Cells were loaded according to standard protocol of the Chromium single cell 3’ kit in order to capture between 5000 cells/chip position (V2 chemistry). All the following steps were performed according to the standard manufacturer protocol. Data were sequenced on Illumina HiSeqXTen instruments using 150 nt paired-end sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2019-07-09 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR241800 |
CRR241800_f1.fastq.gz
CRR241800_r2.fastq.gz
|
38,152.59
38,437.13
|
|
Submitter | sun baofa (sunbf@big.ac.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2018-10-13 |