Experiment information
Accession CRX030773
Organism Homo sapiens
Title T12_single cell RNAseq
BioProject PRJCA001063
BioSample SAMC047083
Platform Illumina HiSeq X Ten
Library name Construction protocol Strategy Source Selection Layout
The concentration of single cell suspension was counted using Countess (Thermo) and adjusted to 1000 cells/ul. Cells were loaded according to standard protocol of the Chromium single cell 3’ kit in order to capture between 5000 cells/chip position (V2 chemistry). All the following steps were performed according to the standard manufacturer protocol. Data were sequenced on Illumina HiSeqXTen instruments using 150 nt paired-end sequencing. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-07-09
Run accession Run data file information
File nameFile size (MB)
CRR034507 CRR034507_f1.fastq.gz
Submittersun baofa (sunbf@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-10-13