| Accession | CRX030782 |
| Organism | Homo sapiens |
| Title | T21_single cell RNAseq |
| BioProject | PRJCA001063 |
| BioSample | SAMC047092 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The concentration of single cell suspension was counted using Countess (Thermo) and adjusted to 1000 cells/ul. Cells were loaded according to standard protocol of the Chromium single cell 3’ kit in order to capture between 5000 cells/chip position (V2 chemistry). All the following steps were performed according to the standard manufacturer protocol. Data were sequenced on Illumina HiSeqXTen instruments using 150 nt paired-end sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2019-07-09 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR034516 |
CRR034516_f1.fastq.gz
CRR034516_r2.fastq.gz
|
43,548.03
41,409.46
|
|
| Submitter | sun baofa (sunbf@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-10-13 |
|---|
