| 实验编号 | CRX030785 |
| 物种名称 | Homo sapiens |
| 标题 | T24_single cell RNAseq |
| 项目编号 | PRJCA001063 |
| 样本编号 | SAMC047095 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The concentration of single cell suspension was counted using Countess (Thermo) and adjusted to 1000 cells/ul. Cells were loaded according to standard protocol of the Chromium single cell 3’ kit in order to capture between 5000 cells/chip position (V2 chemistry). All the following steps were performed according to the standard manufacturer protocol. Data were sequenced on Illumina HiSeqXTen instruments using 150 nt paired-end sequencing. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| 发布日期 | 2019-07-09 |
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
| CRR034519 |
CRR034519_f1.fastq.gz
CRR034519_r2.fastq.gz
|
38,836.33
41,486.47
|
|
| 提交者 | sun baofa (sunbf@big.ac.cn) |
| 所属单位 | Beijing Institute of Genomics, Chinese Academy of Sciences |
| 提交日期 | 2018-10-13 |