| 实验编号 | CRX062337 |
| 物种名称 | Homo sapiens |
| 标题 | Jurkat-1-IP |
| 项目编号 | PRJCA001180 |
| 样本编号 | SAMC106570 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total RNA was extracted from tissues using TRIzol reagent (Invitrogen,15596018), followed by DNase I (NEB, M0303L) treatment to remove DNA contamination. Additional phenol-chloroform isolation and ethanol precipitation treatment was performed to remove enzyme contamination. total RNA was fragmented into ~130-nucleotide-long fragments by magnesium RNA fragmentation buffer (NEB, E6150S). The fragmentation was stopped by adding RNA fragmentation stop solution followed by ethanol precipitation. 8ng of fragmented total RNA was used as input and remained RNA was used to do the m6A-seq. The libraries were sequenced on Illumina Hiseq X10 with paired-end 2X 150 bp read length. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 130
|
| 发布日期 | 2020-01-08 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR073013 |
CRR073013_f1.fastq.gz
CRR073013_r2.fastq.gz
|
7,547.06
8,132.31
|
|
| 提交者 | li kai (li_kai@pku.edu.cn) |
|---|
| 所属单位 | Peking University |
|---|
| 提交日期 | 2019-09-12 |
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