| 实验编号 | CRX101341 |
| 物种名称 | Homo sapiens |
| 标题 | RNA-seq of PBMC: healthy donor 2 |
| 项目编号 | PRJCA002326 |
| 样本编号 | SAMC149135 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
One microgram of total RNAs extracted from PBMCs (Ficoll preparation) were used as input. Messenger RNAs were purified using oligo-dTs covalently coupled magnetic beads. Then, the RNAs were fragmented into small pieces by heating. First strand of cDNA was synthesized in the presence of specific chemicals to ensure that only RNAs were used as template. Double strand cDNAs were purified with Agencourt AMPure XP beads after the reaction. DNA library was constructed through end-repair, adaptor-ligation and PCR amplification. The intermediate products were size-selected after the adaptor-ligation using two rounds of Agencourt AMPure XP beads. Qualified double strand DNA library was transformed into single-stranded circular DNA library through DNA-denaturation and circularization. DNA nanoballs (DNBs) were generated from single-stranded circular DNA using rolling circle amplification (RCA). The DNBs were qualified using Qubit 2.0. Qualified DNBs were loaded on the flow cell and sequenced. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 145
|
| 发布日期 | 2020-04-01 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR125445 |
CRR125445_f1.fastq.gz
CRR125445_r2.fastq.gz
|
3,682.52
3,836.07
|
|
| 提交者 | Yu Zhou (yu.zhou@whu.edu.cn) |
|---|
| 所属单位 | Wuhan University |
|---|
| 提交日期 | 2020-03-18 |
|---|
