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实验基本信息
实验编号 CRX144841
物种名称 Mus musculus
标题 3SS_12h_Control_1_19
项目编号 PRJCA003264
样本编号 SAMC221743
测序平台 Illumina HiSeq 4000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
cDNA from 48-96 different cells was pooled together. Four-cycle PCR using biotinylated index primers introduced a biotin tag to the 3’ end of the cDNA. After cDNA fragmentation using Bioruptor plus (Diagenode), the 3’ terminals of the cDNA were captured using Dynabeads MyOne Streptavidin C1 (Thermo, 65002). Thirty nanograms of cDNA was used to construct the libraries using a Kapa Hyper Prep Kit (Kapa, KK8505) RNA-Seq TRANSCRIPTOMIC PCR SINGLE
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 425
Planned read length (bp): 150
发布日期2020-11-11
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR170910 CRR170910.fq.gz 5.89
提交者Cheng-Ran Xu (cxu@pku.edu.cn)
所属单位Peking University
提交日期2020-08-17
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