| 实验编号 | CRX145136 |
| 物种名称 | Mus musculus |
| 标题 | 3SS_12h_JK184_20umol_1_46 |
| 项目编号 | PRJCA003264 |
| 样本编号 | SAMC221901 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
cDNA from 48-96 different cells was pooled together. Four-cycle PCR using biotinylated index primers introduced a biotin tag to the 3’ end of the cDNA. After cDNA fragmentation using Bioruptor plus (Diagenode), the 3’ terminals of the cDNA were captured using Dynabeads MyOne Streptavidin C1 (Thermo, 65002). Thirty nanograms of cDNA was used to construct the libraries using a Kapa Hyper Prep Kit (Kapa, KK8505) |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
SINGLE
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 425
Planned read length (bp): 150
|
| 发布日期 | 2020-11-11 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR171210 |
CRR171210.fq.gz
|
65.68
|
|
| 提交者 | Cheng-Ran Xu (cxu@pku.edu.cn) |
|---|
| 所属单位 | Peking University |
|---|
| 提交日期 | 2020-08-17 |
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