| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA of adult earthworms was extracted using TRIzol reagent16, while mRNA was isolated using the kit PolyATtract mRNA Isolation System III (Promega, Madison, WI, USA). A total of 1.5 μg RNA per sample was used as the input material for preparation of individual RNA libraries. Libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Beverly, MA, USA) following the manufacturer’s recommendations. Polymerase chain reaction (PCR) products with insert sizes of 150-200 bp were purified using the AMPure XP system and library quality was assessed with the Agilent Bioanalyzer 2100 system. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
