| 实验编号 | CRX165643 |
| 物种名称 | Bacteria |
| 标题 | M133_16S |
| 项目编号 | PRJCA003568 |
| 样本编号 | SAMC250431 |
| 测序平台 | Illumina MiSeq |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
DNA was extracted using a MagNA Pure LC 2.0 system and a MagNA Pure LC Total NA Isolation kit in accordance with the manufacturer’s instructions and quantified using a QuantiT PicoGreen dsDNA Assay kit. PCR amplification of the V3-V4 region was performed with the following primers containing Illumina adapter sequences and dual-index barcodes to tag each sample. The amplicon sequencing libraries were constructed in accordance with the 16S Metagenomic Sequencing Library Preparation. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
| 发布日期 | 2021-05-13 |
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
| CRR193520 |
CRR193520_f1.fq.gz
CRR193520_r2.fq.gz
|
0.04
0.05
|
|
| 提交者 | Jiarui Li (li.jiarui@ccmu.edu.cn) |
| 所属单位 | Beijing Ditan Hospital, Capital Medical University |
| 提交日期 | 2020-09-30 |