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实验基本信息
实验编号 CRX165643
物种名称 Bacteria
标题 M133_16S
项目编号 PRJCA003568
样本编号 SAMC250431
测序平台 Illumina MiSeq
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
DNA was extracted using a MagNA Pure LC 2.0 system and a MagNA Pure LC Total NA Isolation kit in accordance with the manufacturer’s instructions and quantified using a QuantiT PicoGreen dsDNA Assay kit. PCR amplification of the V3-V4 region was performed with the following primers containing Illumina adapter sequences and dual-index barcodes to tag each sample. The amplicon sequencing libraries were constructed in accordance with the 16S Metagenomic Sequencing Library Preparation. AMPLICON GENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
发布日期2021-05-13
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR193520 CRR193520_f1.fq.gz
CRR193520_r2.fq.gz
0.04
0.05
提交者Jiarui Li (li.jiarui@ccmu.edu.cn)
所属单位Beijing Ditan Hospital, Capital Medical University
提交日期2020-09-30