Accession | CRX165655 |
Organism | Bacteria |
Title | M420_16S |
BioProject | PRJCA003568 |
BioSample | SAMC250443 |
Platform | Illumina MiSeq |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA was extracted using a MagNA Pure LC 2.0 system and a MagNA Pure LC Total NA Isolation kit in accordance with the manufacturer’s instructions and quantified using a QuantiT PicoGreen dsDNA Assay kit. PCR amplification of the V3-V4 region was performed with the following primers containing Illumina adapter sequences and dual-index barcodes to tag each sample. The amplicon sequencing libraries were constructed in accordance with the 16S Metagenomic Sequencing Library Preparation. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
Release date | 2021-05-13 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR193532 |
CRR193532_f1.fq.gz
CRR193532_r2.fq.gz
|
0.72
0.92
|
|
Submitter | Jiarui Li (li.jiarui@ccmu.edu.cn) |
Organization | Beijing Ditan Hospital, Capital Medical University |
Date submitted | 2020-09-30 |