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Experiment information
Accession CRX165670
Organism Bacteria
Title M014_16S
BioProject PRJCA003568
BioSample SAMC250458
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
DNA was extracted using a MagNA Pure LC 2.0 system and a MagNA Pure LC Total NA Isolation kit in accordance with the manufacturer’s instructions and quantified using a QuantiT PicoGreen dsDNA Assay kit. PCR amplification of the V3-V4 region was performed with the following primers containing Illumina adapter sequences and dual-index barcodes to tag each sample. The amplicon sequencing libraries were constructed in accordance with the 16S Metagenomic Sequencing Library Preparation. AMPLICON GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Release date2021-05-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR193547 CRR193547_f1.fq.gz
CRR193547_r2.fq.gz
0.18
0.22
SubmitterJiarui Li (li.jiarui@ccmu.edu.cn)
OrganizationBeijing Ditan Hospital, Capital Medical University
Date submitted2020-09-30