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Experiment information
Accession CRX446363
Organism Mus musculus
Title msday7control
BioProject PRJCA004238
BioSample SAMC784992
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
scRNA-seq libraries were generated utilizing the Chromium Single Cell 5’ Library and Gel Bead Kit (10x Genomics, 120237) according to the manufacturer’s instruction with some modifications. In detail, after washing with 0.04% BSA buffer (0.02 g BSA dissolved in 50 ml deionized PBS), lymph node cells of normal mice were captured in droplets. Then, reverse transcription, emulsion breaking, barcoded-cDNA purification with Dynabeads, and PCR amplification were conducted step by step. The amplified cDNA was then used for 5’ gene expression library construction. Specifically, fragmenting and end-repair, double-size selection with SPRIselect beads, and sequencing were conducted on 50 ng of amplified cDNA using NovaSeq platform (Illumina NovaSeq6000) to yield 150 bp paired-end reads. RNA-Seq TRANSCRIPTOMIC SINGLE CELL other PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-08-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR505967 CRR505967_f1.fastq.gz
CRR505967_r2.fastq.gz 		2,031.31
7,692.02
CRR505968 CRR505968_f1.fastq.gz
CRR505968_r2.fastq.gz 		1,733.67
6,550.63
CRR505969 CRR505969_f1.fastq.gz
CRR505969_r2.fastq.gz 		2,321.37
8,714.21
CRR505970 CRR505970_f1.fastq.gz
CRR505970_r2.fastq.gz 		1,556.99
5,856.31
CRR505971 CRR505971_f1.fastq.gz
CRR505971_r2.fastq.gz 		144.05
529.25
CRR505972 CRR505972_f1.fastq.gz
CRR505972_r2.fastq.gz 		123.62
453.04
CRR505973 CRR505973_f1.fastq.gz
CRR505973_r2.fastq.gz 		164.85
601.42
CRR505974 CRR505974_f1.fastq.gz
CRR505974_r2.fastq.gz 		110.17
403.15
CRR505975 CRR505975_f1.fastq.gz
CRR505975_r2.fastq.gz 		222.41
834.23
CRR505976 CRR505976_f1.fastq.gz
CRR505976_r2.fastq.gz 		189.93
711.05
CRR505977 CRR505977_f1.fastq.gz
CRR505977_r2.fastq.gz 		248.32
927.53
CRR505978 CRR505978_f1.fastq.gz
CRR505978_r2.fastq.gz 		170.04
633.22
SubmitterWenru Su (suwr3@mail.sysu.edu.cn)
OrganizationZhongshan Ophthalmic Center,Sun Yat-sen University
Date submitted2022-05-27
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