| 实验编号 | CRX446365 |
| 物种名称 | Mus musculus |
| 标题 | msday21control |
| 项目编号 | PRJCA004238 |
| 样本编号 | SAMC784994 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
scRNA-seq libraries were generated utilizing the Chromium Single Cell 5’ Library and Gel Bead Kit (10x Genomics, 120237) according to the manufacturer’s instruction with some modifications. In detail, after washing with 0.04% BSA buffer (0.02 g BSA dissolved in 50 ml deionized PBS), lymph node cells of IL-38-treated EAU mice were captured in droplets. Then, reverse transcription, emulsion breaking, barcoded-cDNA purification with Dynabeads, and PCR amplification were conducted step by step. The amplified cDNA was then used for 5’ gene expression library construction. Specifically, fragmenting and end-repair, double-size selection with SPRIselect beads, and sequencing were conducted on 50 ng of amplified cDNA using NovaSeq platform (Illumina NovaSeq6000) to yield 150 bp paired-end reads. |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
other |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2022-08-11 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR505987 |
CRR505987_f1.fastq.gz
CRR505987_r2.fastq.gz
|
2,218.2
8,354.24
|
| CRR505988 |
CRR505988_f1.fastq.gz
CRR505988_r2.fastq.gz
|
2,966.3
11,399.11
|
| CRR505989 |
CRR505989_f1.fastq.gz
CRR505989_r2.fastq.gz
|
1,976.48
7,539.66
|
| CRR505990 |
CRR505990_f1.fastq.gz
CRR505990_r2.fastq.gz
|
2,228.26
8,428.37
|
|
| 提交者 | Wenru Su (suwr3@mail.sysu.edu.cn) |
|---|
| 所属单位 | Zhongshan Ophthalmic Center,Sun Yat-sen University |
|---|
| 提交日期 | 2022-05-27 |
|---|
