Experiment information
Accession CRX446365
Organism Mus musculus
Title msday21control
BioProject PRJCA004238
BioSample SAMC784994
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
scRNA-seq libraries were generated utilizing the Chromium Single Cell 5’ Library and Gel Bead Kit (10x Genomics, 120237) according to the manufacturer’s instruction with some modifications. In detail, after washing with 0.04% BSA buffer (0.02 g BSA dissolved in 50 ml deionized PBS), lymph node cells of IL-38-treated EAU mice were captured in droplets. Then, reverse transcription, emulsion breaking, barcoded-cDNA purification with Dynabeads, and PCR amplification were conducted step by step. The amplified cDNA was then used for 5’ gene expression library construction. Specifically, fragmenting and end-repair, double-size selection with SPRIselect beads, and sequencing were conducted on 50 ng of amplified cDNA using NovaSeq platform (Illumina NovaSeq6000) to yield 150 bp paired-end reads. RNA-Seq TRANSCRIPTOMIC SINGLE CELL other PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-08-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR505987 CRR505987_f1.fastq.gz
CRR505987_r2.fastq.gz
2,218.2
8,354.24
CRR505988 CRR505988_f1.fastq.gz
CRR505988_r2.fastq.gz
2,966.3
11,399.11
CRR505989 CRR505989_f1.fastq.gz
CRR505989_r2.fastq.gz
1,976.48
7,539.66
CRR505990 CRR505990_f1.fastq.gz
CRR505990_r2.fastq.gz
2,228.26
8,428.37
SubmitterWenru Su (suwr3@mail.sysu.edu.cn)
OrganizationZhongshan Ophthalmic Center,Sun Yat-sen University
Date submitted2022-05-27