Accession | CRX220375 |
Organism | Oryza sativa Japonica Group |
Title | rice_rep1_nanopore |
BioProject | PRJCA004326 |
BioSample | SAMC311671 |
Platform | OXFORD_NANOPORE PromethION |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The extracted genomic DNA was qualified, size-selected using the BluePippin system (Sage Science). Then, the genomic DNA was end-repaired and PCR adapters supplied in ONT sequencing kit (SQK-LSK109) were ligated to the end-repaired DNA. Finally, Qubit® 3.0 Fluorometer (Invitrogen) was used to quantify the size of library fragments. To generate Nanopore reads, the prepared libraries are loaded into flow cells (R9.4, FLO-PRO002) of a PromethION sequencer (ONT). |
WGS |
GENOMIC |
unspecified |
SINGLE
|
|
Processing |
Planned read length (bp): 25990
|
Release date | 2021-02-09 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR261442 |
CRR261442.tar.gz
|
1,122,713.26
|
|
Submitter | Peng Ni (nipeng@csu.edu.cn) |
Organization | Central South University |
Date submitted | 2021-02-05 |