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Single cell RNA-seq was performed using the Single Cell 5' RNA Reagent Kits (10x Genomics, Pleasanton, California) according to the manufacturer’s instruction.Cellular suspensions were loaded on the sample chip in the Chromium Controller instrument (10X Genomics) to generate single-cell Gel Bead-In-Emulsions (GEMs). After RT, GEMs were harvested and the cDNAs were amplified, and cleaned up with SPRIselect Reagent Kit. libraries were constructed using Chromium Single Cell 5' Library kit based for enzymatic fragmentation, end-repair, A-tailing, adaptor ligation, ligation cleanup, sample index PCR, and PCR cleanup. |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
PCR |
PAIRED
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