| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The scATAC library was prepared using the 10x Genomics platform with the Chromium Single Cell ATAC Library & Gel Bead Kit (10x Genomics, Pleasanton, California) as instructed by the manufacturer. A total of 15,000 nuclei per sample were used as input for single-cell ATAC-seq following the manufacturer’s instructions. Briefly, after tagmentation, the cells were loaded on a Chromium Controller Single-Cell instrument to generate single-cell Gel Bead-In-Emulsions (GEMs) followed by linear PCR as described in the 10X scATAC-seq protocol using a Veriti 96-well thermal cycler (BioRad, 1851197). After breaking the GEMs, the barcoded tagmented DNA was purified with SPRIselect Reagent Kit (Beckman Coulter, Pasadena, CA) and further amplified to enable sample indexing and enrichment of scATAC-seq libraries. |
ATAC-seq |
GENOMIC SINGLE CELL |
PCR |
PAIRED
|
|
