| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Single cell was transferred into prepared lysis buffer contained an 8bp barcode. Then, the first-strand cDNA was reversed-synthesized and amplified in a reverse transcription (RT) mixture containing 4U RNase inhibitor, 100U SuperScript II reverse transcriptase (Invitrogen, Cat. 18064071), 1 mM dNTPs (TAKARA, Cat. 4019), 60 mM MgCl2, 3 µM RT primer with 10 µM TSO primer. After PCR incubation, the product was purified by 0.8×AMPure XP beads (Beckman, Cat. A63882). Biotin PCR was carried out and enriched. The single cell RNA-seq library was constructed according to the KAPA Hyper Prep Kits with PCR Library Amplication/Illumina series (KAPA, KK8054). |
ssRNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
RANDOM |
PAIRED
|
|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR271345 |
CRR271345_f1.fq.gz
CRR271345_r2.fq.gz
|
15,744.23
15,013.01
|
| CRR271346 |
CRR271346_f1.fq.gz
CRR271346_r2.fq.gz
|
12,221.44
13,404.43
|
| CRR271347 |
CRR271347_f1.fq.gz
CRR271347_r2.fq.gz
|
11,695.23
12,930.62
|
| CRR271348 |
CRR271348_f1.fq.gz
CRR271348_r2.fq.gz
|
16,727.25
16,883.13
|
| CRR271349 |
CRR271349_f1.fq.gz
CRR271349_r2.fq.gz
|
18,689.25
19,659.22
|
| CRR271350 |
CRR271350_f1.fq.gz
CRR271350_r2.fq.gz
|
14,165.64
14,875.28
|
| CRR271351 |
CRR271351_f1.fq.gz
CRR271351_r2.fq.gz
|
13,296.67
15,170.82
|
| CRR271352 |
CRR271352_f1.fq.gz
CRR271352_r2.fq.gz
|
14,415.55
15,703.76
|
| CRR271353 |
CRR271353_f1.fq.gz
CRR271353_r2.fq.gz
|
13,403.91
14,407.07
|
| CRR271354 |
CRR271354_f1.fq.gz
CRR271354_r2.fq.gz
|
14,893.84
15,423.99
|
| CRR271355 |
CRR271355_f1.fq.gz
CRR271355_r2.fq.gz
|
13,703.87
13,527.85
|
| CRR271356 |
CRR271356_f1.fq.gz
CRR271356_r2.fq.gz
|
15,923.27
15,460.14
|
| CRR271357 |
CRR271357_f1.fq.gz
CRR271357_r2.fq.gz
|
14,761.01
14,929.12
|
| CRR271358 |
CRR271358_f1.fq.gz
CRR271358_r2.fq.gz
|
12,396.03
12,289.85
|
| CRR271359 |
CRR271359_f1.fq.gz
CRR271359_r2.fq.gz
|
17,459.01
17,136.78
|
| CRR271360 |
CRR271360_f1.fq.gz
CRR271360_r2.fq.gz
|
15,560.96
16,954.56
|
| CRR271361 |
CRR271361_f1.fq.gz
CRR271361_r2.fq.gz
|
18,701.66
20,631.76
|
| CRR271362 |
CRR271362_f1.fq.gz
CRR271362_r2.fq.gz
|
13,279.81
14,001.09
|
| CRR271363 |
CRR271363_f1.fq.gz
CRR271363_r2.fq.gz
|
15,186.69
16,065.7
|
| CRR271364 |
CRR271364_f1.fq.gz
CRR271364_r2.fq.gz
|
10,127.82
11,635.33
|
|
