| Accession | CRX228983 |
| Organism | Sus scrofa |
| Title | bulk RNA-sequencing of pluripotent stem cell lines from porcine E10 pre-gastrulation epiblasts(pgEpiSCs) |
| BioProject | PRJCA004466 |
| BioSample | SAMC341725 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
total RNAs were extracted using the RNeasy Mini Kit (Qiagen, Cat.N: 74106). We used an rRNA depletion protocol (Globin-Zero Gold rRNA Removal Kit, Illumina, Cat.N: GZG1224) coupled with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Cat.N: E7420S) to construct the strand-specific RNA-seq library for each sample. All libraries were quantified using the Qubit dsDNA High Sensitivity Assay Kit (Invitrogen, Life Technologies, Cat.N: Q32851) and sequenced on HiSeq 4000 platform (Illumina) |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2021-12-02 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR271367 |
CRR271367_f1.fq.gz
CRR271367_r2.fq.gz
|
3,179.68
3,297.04
|
| CRR271368 |
CRR271368_f1.fq.gz
CRR271368_r2.fq.gz
|
4,197.65
4,424.97
|
| CRR271369 |
CRR271369_f1.fq.gz
CRR271369_r2.fq.gz
|
4,790.04
4,956.46
|
| CRR271370 |
CRR271370_f1.fq.gz
CRR271370_r2.fq.gz
|
4,107.16
4,262.58
|
|
| Submitter | Caihong Zheng (zhengch@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2021-02-26 |
|---|
