| Accession | CRX228987 |
| Organism | Sus scrofa |
| Title | ChIP-sequencing of pluripotent stem cell lines from porcine E10 pre-gastrulation epiblasts(pgEpiSCs) |
| BioProject | PRJCA004466 |
| BioSample | SAMC341725 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Cells were lysed with lysis buffer supplemented with protease inhibitor cocktail and 1 mM PMSF (1x final each) and then sonicated to fragments about 200-500 bp using a Bioruptor. Immunoprecipitated DNA was isolated with H3K27ac antibodies (Abcam, Cat.N: ab4729). Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the NEXTflex™ ChIP-Seq Kit (Bioo Scientific, Cat.N: NOVA-5143-02). All libraries were sequenced on HiSeq X Ten (Illumina) platform to produce an average of ~120.62 million 150-bp paired-end raw reads and ~115.78 million high-quality reads for each library. |
ChIP-Seq |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2021-12-02 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR271405 |
CRR271405_f1.fastq.gz
CRR271405_r2.fastq.gz
|
3,939.42
3,988.48
|
| CRR271406 |
CRR271406_f1.fastq.gz
CRR271406_r2.fastq.gz
|
4,439.31
4,486.7
|
| CRR271407 |
CRR271407_f1.fastq.gz
CRR271407_r2.fastq.gz
|
5,106.02
5,193.29
|
| CRR271408 |
CRR271408_f1.fastq.gz
CRR271408_r2.fastq.gz
|
4,629.72
4,749.5
|
|
| Submitter | Caihong Zheng (zhengch@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2021-02-26 |
|---|
