| Accession | CRX228988 |
| Organism | Sus scrofa |
| Title | ChIP-sequencing of embryonic fibroblast from porcine E30 fetals(pEFs) |
| BioProject | PRJCA004466 |
| BioSample | SAMC341726 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Cells were lysed with lysis buffer supplemented with protease inhibitor cocktail and 1 mM PMSF (1x final each) and then sonicated to fragments about 200-500 bp using a Bioruptor. Immunoprecipitated DNA was isolated with H3K27ac antibodies (Abcam, Cat.N: ab4729). Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the NEXTflex™ ChIP-Seq Kit (Bioo Scientific, Cat.N: NOVA-5143-02). All libraries were sequenced on HiSeq X Ten (Illumina) platform to produce an average of ~120.62 million 150-bp paired-end raw reads and ~115.78 million high-quality reads for each library. |
ChIP-Seq |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2021-12-02 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR271409 |
CRR271409_f1.fastq.gz
CRR271409_r2.fastq.gz
|
4,319.66
4,342.79
|
| CRR271410 |
CRR271410_f1.fastq.gz
CRR271410_r2.fastq.gz
|
4,357.03
4,387.64
|
| CRR271411 |
CRR271411_f1.fastq.gz
CRR271411_r2.fastq.gz
|
4,087.79
4,221.28
|
| CRR271412 |
CRR271412_f1.fastq.gz
CRR271412_r2.fastq.gz
|
3,772.83
3,842.48
|
|
| Submitter | Caihong Zheng (zhengch@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2021-02-26 |
|---|
