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Experiment information
Accession CRX235971
Organism Oryza sativa
Title ID-1
BioProject PRJCA004855
BioSample SAMC350951
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
We constructed scRNA-seq libraries from isolated protoplasts, following the manufacturer's protocol of the 10x Genomics Single Cell 3' Reagent Kits v3. Protoplasts were loaded onto the 10x Genomics Chromium single cell microfluidics device. PCR was performed for 11 cycles to amplify the pooled complement DNA. After ligating to the sequencing adapters, complement DNA was again amplified by PCR for 10~15 cycles, according to the DNA concentration of the template. scRNA-seq libraries were sequenced on the Illumina NovaSeq 6000 platform in the paired-end 150-nt mode. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-06-18
Run
Run accession Run data file information
File nameFile size (MB)
CRR279115 CRR279115.tar 19,779.72
CRR279116 CRR279116.tar 19,258.38
CRR279117 CRR279117.tar 17,834.58
CRR279118 CRR279118.tar 22,870.93
CRR279119 CRR279119.tar 2,351.08
CRR279120 CRR279120.tar 2,255.19
CRR279121 CRR279121.tar 2,098.27
CRR279122 CRR279122.tar 2,703.39
SubmitterQing Huan (qhuan@genetics.ac.cn)
OrganizationInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
Date submitted2021-04-16
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