logo
  1. Home
  2. Browse
  3. CRA004082
  4. CRX235973
Experiment information
Accession CRX235973
Organism Oryza sativa
Title LN-2
BioProject PRJCA004855
BioSample SAMC350953
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
We constructed scRNA-seq libraries from isolated protoplasts, following the manufacturer's protocol of the 10x Genomics Single Cell 3' Reagent Kits v3. Protoplasts were loaded onto the 10x Genomics Chromium single cell microfluidics device. PCR was performed for 11 cycles to amplify the pooled complement DNA. After ligating to the sequencing adapters, complement DNA was again amplified by PCR for 10~15 cycles, according to the DNA concentration of the template. scRNA-seq libraries were sequenced on the Illumina NovaSeq 6000 platform in the paired-end 150-nt mode. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-06-18
Run
Run accession Run data file information
File nameFile size (MB)
CRR279127 CRR279127.tar 32,511.88
CRR279128 CRR279128.tar 45,568.21
CRR279129 CRR279129.tar 35,978.42
CRR279130 CRR279130.tar 38,353.05
CRR279131 CRR279131.tar 686.68
CRR279132 CRR279132.tar 969.2
CRR279133 CRR279133.tar 763.28
CRR279134 CRR279134.tar 812.13
SubmitterQing Huan (qhuan@genetics.ac.cn)
OrganizationInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
Date submitted2021-04-16
Related experiments
Experiments(27)