| Accession | CRX235986 |
| Organism | mixed sample |
| Title | Post-protoplasting replicate 1 |
| BioProject | PRJCA004855 |
| BioSample | SAMC350966 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We constructed scRNA-seq libraries from isolated protoplasts, following the manufacturer's protocol of the 10x Genomics Single Cell 3' Reagent Kits v3. Protoplasts were loaded onto the 10x Genomics Chromium single cell microfluidics device. PCR was performed for 11 cycles to amplify the pooled complement DNA. After ligating to the sequencing adapters, complement DNA was again amplified by PCR for 10~15 cycles, according to the DNA concentration of the template. scRNA-seq libraries were sequenced on the Illumina NovaSeq 6000 platform in the paired-end 150-nt mode. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2021-06-18 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR279177 |
CRR279177_f1.fastq.gz
CRR279177_r2.fastq.gz
|
1,662.69
1,848.76
|
|
| Submitter | Qing Huan (qhuan@genetics.ac.cn) |
|---|
| Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
|---|
| Date submitted | 2021-04-16 |
|---|
