| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Following the standard protocol described previously with certain modifications (Belt
on et al., 2012), samples were flash frozen and pulverized prior to formaldehyde
crosslinking. After the completion of the sample of crosslinking, cell lysis, using restri
ction enzymes (DPNII) digestion, and sampling detection enzyme diges
tion. After that, the ends were labeled by biotin and then supplemented and connected.
Protease K and SDS were added to uncross linked. DNA was purified and extracted b
y Ampure XP beads, and then sampled detection DNA quality. After passing the test,
the standard library construction process would start. DNA fragments with terminal m
arkers but not connected were removed. Ultrasonically interrupted to 200-500bp. The
biotin-labeled DNA fragments were captured by M-280 Streptavidin beads. The ter
minal was repaired by adding with A-tails and then added the sequencing connector. T
hen obtained the library product by PCR. Finally, Illumina HiSeq was used for sequen
cing after the constructed library was qualified by library quality control. |
Hi-C |
GENOMIC |
Restriction Digest |
PAIRED
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