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About 7000 cells were loaded onto 10x Chromium chips with 3v2 chemistry and barcode to achieve a targeted cell count of 4000, according to the manufacturers instructions. After cDNA synthesis, 14 amplification cycles were carried out for each library preparation. The resultant libraries were sequenced using 2 150 paired-end sequencing protocol on an Illumina NovaSeq 6000 platform, with a read length of 26 bp for cell barcode and unique molecule identifier (UMI) (read 1), 8 bp i7 index read (sample barcode), and 98 bp for actual RNA read (read 2). Each biosample yielded about 550M reads. |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
cDNA |
PAIRED
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