| 实验编号 | CRX280906 |
| 物种名称 | Mus musculus |
| 标题 | SKOM+Flag-D5H3K27ac |
| 项目编号 | PRJCA006785 |
| 样本编号 | SAMC464010 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols. |
ChIP-Seq |
GENOMIC |
ChIP |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2022-11-13 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR328492 |
CRR328492_f1.fq.gz
CRR328492_r2.fq.gz
|
1,091.02
1,126.54
|
|
| 提交者 | Li Wei (huitu09@163.com) |
|---|
| 所属单位 | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences |
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| 提交日期 | 2021-10-06 |
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