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Experiment information
Accession CRX289311
Organism Populus euphratica
Title F-30-E
BioProject PRJCA006811
BioSample SAMC477196
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
A total amount of 5.2 µg DNA spiked with 26 ng lambda DNA fragmented, followed by end repair and adenylation, ligated with cytosine-methylated barcodes following manufacturer’s instructions, treated twice with bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research), and the resulting single-strand DNA fragments were PCR amplified using KAPA HiFi HotStart Uracil + ReadyMix (2X). Bisulfite-Seq GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-10-05
Run
Run accession Run data file information
File nameFile size (MB)
CRR337198 CRR337198_f1.fq.gz
CRR337198_r2.fq.gz
4,315.01
4,585.91
SubmitterShanhe Zhang (zhanghh1127@163.com)
OrganizationTarim university
Date submitted2021-11-04