Accession | CRX289314 |
Organism | Populus euphratica |
Title | M-9-E |
BioProject | PRJCA006811 |
BioSample | SAMC477199 |
Platform | Illumina NovaSeq 6000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 5.2 µg DNA spiked with 26 ng lambda DNA fragmented, followed by end repair and adenylation, ligated with cytosine-methylated barcodes following manufacturer’s instructions, treated twice with bisulfite using EZ DNA Methylation-Gold Kit (Zymo Research), and the resulting single-strand DNA fragments were PCR amplified using KAPA HiFi HotStart Uracil + ReadyMix (2X). |
Bisulfite-Seq |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2022-10-05 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR337201 |
CRR337201_f1.fq.gz
CRR337201_r2.fq.gz
|
3,297.08
3,380.25
|
|
Submitter | Shanhe Zhang (zhanghh1127@163.com) |
Organization | Tarim university |
Date submitted | 2021-11-04 |