logo
  1. Home
  2. Browse
  3. CRA005430
  4. CRX294232
实验基本信息
实验编号 CRX294232
物种名称 Alsophila spinulosa
标题 small_RNA-2
项目编号 PRJCA006485
样本编号 SAMC489744
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
We used magnetic beads with Oli (dT) to enrich the mRNA and broke it into pieces by applying a fragmentation buffer. Employing these mRNAs as the template, random hexamers were used to synthesize the first-strand cDNA. Next, together with buffer, the dNTPs, RNaseH, and DNA polymerase I synthesized the second-strand cDNA. Each cDNA library was purified and to it a joint end with A was added. OTHER GENOMIC PCR SINGLE
处理信息 Planned read length (bp): 50
发布日期2022-03-18
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR342771 CRR342771.fq.gz 260.5
提交者xiong huang (huangxiongcaf@163.com)
所属单位Chinese Academy of Forestry
提交日期2021-11-23
关联实验
Experiments(5)