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For PacBio sequencing, DNA was sheared into about 10 Kb by G-tub and purified by AMPure PB magnetic beads. The quality of the fragmented DNA was detected by 0.7% agarose gel electrophoresis and qualified samples were used to generate the sequencing library according to the manufacturer's instructions of SMRTbellTM Express Template Prep Kit 2.0 (Pacific Biosciences, USA). The quality and quantity of DNA libraries were then checked by Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and then sequenced by PacBio RS II sequencer (Pacific Biosciences, Menlo Park, CA, USA). |
WGS |
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PCR |
SINGLE
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