| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA was extracted using a QIAGEN® Genomic DNA extraction kit (Cat#13323, QIAGEN) according to the standard operating procedure provided by the manufacturer. The extracted DNA was detected by a NanoDrop™ One UV-Vis spectrophotometer (Thermo Fisher Scientific, USA) for DNA purity (OD260/280 ranging from 1.8 to 2.0 and OD 260/230 is between 2.0 and 2.2), then Qubit® 3.0 Fluorometer (Invitrogen, USA) was used to quantify DNA accurately. After that, long DNA fragments were selected and collected using the BluePippin system (Sage Science, USA). Recycled DNA fragments were subjected to end repair and adaptor ligation by SQK-LSK109 kit. |
WGS |
GENOMIC |
RANDOM |
SINGLE
|
|
