| Accession | CRX398528 |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Title | mNGS sequencing WH01 |
| BioProject | PRJCA008874 |
| BioSample | SAMC703646 |
| Platform | DNBSEQ-T7 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
140 μL bronchoalveolar lavage fluid samples (WH01 to WH04) were reserved for RNA extraction using the QIAamp Viral RNA Mini Kit (52904; Qiagen, Heiden, Germany), according to the manufacturer's recommendations. A probe-captured technique was used to remove human nucleic acid. The remaining RNA was reverse-transcribed into cDNA, followed by the second-strand synthesis. Using the synthetic double-stranded DNA, a DNA library was constructed through DNA-fragmentation, end-repair, adaptor-ligation, and PCR amplification. The constructed library was qualified with an Invitrogen Qubit 2.0 Fluorometer (ThermoFisher, Foster City, CA, USA), and the qualified double-stranded DNA library was transformed into a single-stranded circular DNA library through DNA-denaturation and circularisation. DNA nanoballs were generated from single-stranded circular DNA by rolling circle amplification, then qualified with Qubit 2.0 and loaded onto the flow cell and sequenced with PE100 on the DNBSEQ-T7 platform (MGI, Shenzhen, China). |
OTHER |
METATRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-04-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR456574 |
CRR456574_f1.fastq.gz
CRR456574_r2.fastq.gz
|
26,397.28
33,663.09
|
| CRR456575 |
CRR456575_f1.fastq.gz
CRR456575_r2.fastq.gz
|
17,303.82
23,562.56
|
| CRR456576 |
CRR456576_f1.fastq.gz
CRR456576_r2.fastq.gz
|
12,046.19
15,784.92
|
| CRR456577 |
CRR456577_f1.fastq.gz
CRR456577_r2.fastq.gz
|
15,544.06
20,618.6
|
| CRR456578 |
CRR456578_f1.fastq.gz
CRR456578_r2.fastq.gz
|
45,194.12
57,750.74
|
| CRR456579 |
CRR456579_f1.fastq.gz
CRR456579_r2.fastq.gz
|
9,014.89
9,996.55
|
| CRR456580 |
CRR456580_f1.fastq.gz
CRR456580_r2.fastq.gz
|
6,755.44
7,635.37
|
| CRR456581 |
CRR456581_f1.fastq.gz
CRR456581_r2.fastq.gz
|
7,220.47
8,322.64
|
| CRR456582 |
CRR456582_f1.fastq.gz
CRR456582_r2.fastq.gz
|
10,133.37
11,676.05
|
| CRR456583 |
CRR456583_f1.fastq.gz
CRR456583_r2.fastq.gz
|
1,357.94
1,676.35
|
| CRR456584 |
CRR456584_f1.fastq.gz
CRR456584_r2.fastq.gz
|
414.5
589.14
|
| CRR456585 |
CRR456585_f1.fastq.gz
CRR456585_r2.fastq.gz
|
434.19
538.88
|
| CRR456586 |
CRR456586_f1.fastq.gz
CRR456586_r2.fastq.gz
|
795.7
1,070.4
|
| CRR456587 |
CRR456587_f1.fastq.gz
CRR456587_r2.fastq.gz
|
1,644.68
2,063.15
|
|
| Submitter | Changcheng Wu (wucc@ivdc.chinacdc.cn) |
|---|
| Organization | China CDC |
|---|
| Date submitted | 2022-04-07 |
|---|
