| Accession | CRX398529 |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Title | mNGS sequencing WH02 |
| BioProject | PRJCA008874 |
| BioSample | SAMC703647 |
| Platform | DNBSEQ-T7 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
140 μL bronchoalveolar lavage fluid samples (WH01 to WH04) were reserved for RNA extraction using the QIAamp Viral RNA Mini Kit (52904; Qiagen, Heiden, Germany), according to the manufacturer's recommendations. A probe-captured technique was used to remove human nucleic acid. The remaining RNA was reverse-transcribed into cDNA, followed by the second-strand synthesis. Using the synthetic double-stranded DNA, a DNA library was constructed through DNA-fragmentation, end-repair, adaptor-ligation, and PCR amplification. The constructed library was qualified with an Invitrogen Qubit 2.0 Fluorometer (ThermoFisher, Foster City, CA, USA), and the qualified double-stranded DNA library was transformed into a single-stranded circular DNA library through DNA-denaturation and circularisation. DNA nanoballs were generated from single-stranded circular DNA by rolling circle amplification, then qualified with Qubit 2.0 and loaded onto the flow cell and sequenced with PE100 on the DNBSEQ-T7 platform (MGI, Shenzhen, China). |
OTHER |
METATRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-04-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR456588 |
CRR456588_f1.fastq.gz
CRR456588_r2.fastq.gz
|
19,132.58
24,461.25
|
| CRR456589 |
CRR456589_f1.fastq.gz
CRR456589_r2.fastq.gz
|
19,688.9
25,479.73
|
| CRR456590 |
CRR456590_f1.fastq.gz
CRR456590_r2.fastq.gz
|
33,367.61
41,178.38
|
| CRR456591 |
CRR456591_f1.fastq.gz
CRR456591_r2.fastq.gz
|
19,430.27
24,598.64
|
| CRR456592 |
CRR456592_f1.fastq.gz
CRR456592_r2.fastq.gz
|
71,284.37
94,200.21
|
| CRR456593 |
CRR456593_f1.fastq.gz
CRR456593_r2.fastq.gz
|
90,598.86
116,685.54
|
| CRR456594 |
CRR456594_f1.fastq.gz
CRR456594_r2.fastq.gz
|
60,857.57
82,056.61
|
| CRR456595 |
CRR456595_f1.fastq.gz
CRR456595_r2.fastq.gz
|
1,242.65
1,616.19
|
| CRR456596 |
CRR456596_f1.fastq.gz
CRR456596_r2.fastq.gz
|
5,883.56
6,638.76
|
| CRR456597 |
CRR456597_f1.fastq.gz
CRR456597_r2.fastq.gz
|
3,310.29
4,280.22
|
|
| Submitter | Changcheng Wu (wucc@ivdc.chinacdc.cn) |
|---|
| Organization | China CDC |
|---|
| Date submitted | 2022-04-07 |
|---|
